Table 2.

Effects of the C-terminal acidic tail of yeast α subunit on tRNA binding in the context of Encephalitozoon cuniculi γ subunit

				Kd (nM)	Kd (nM)
		Y-α	Encc-γ	Met-tRNAfMet (GDPNP)a	Met-tRNAfMet (GDP)a
1	Encc-eIF2γb	–	wt	1700 ± 300	>24 000
2	ChEncc-eIF2αγ	Wt	wt	300 ± 50	>16 000
3	ChEncc-eIF2(αΔC)γ	αΔC	wt	16 ± 1	3380 ± 550

Dissociation constants were determined from protection experiments as described in ‘Materials and Methods’ section.

^aMeasured with E. coli Met-tRNA_f^Met as a ligand.

^bData from (19).