Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Nov 21;41(2):978–994. doi: 10.1093/nar/gks1078

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

PMC Copyright notice

Figure 2. — Interaction of Drosophila melanogaster GW182 with NOT1, NOT2 and PAN3. (A–G) S2 cells were cotransfected with plasmids expressing GFP-tagged D. melanogaster GW182 (wild-type or mutants) and HA-tagged deadenylase subunits or V5-tagged PABP as indicated. Cell lysates were immunoprecipitated using a polyclonal anti-GFP antibody. GFP-tagged firefly luciferase served as a negative control. Inputs (1%) and immunoprecipitates (5% for GFP-tagged proteins or 40% for HA- or V5-tagged proteins) were analyzed by western blotting using the corresponding antibodies. In all panels, cell lysates were treated with micrococcal nuclease before immunoprecipitation. The presence of endogenous AGO1 in the immunoprecipitates was determined using a specific anti-AGO1 antibody (E).