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. 2012 Nov 21;41(2):978–994. doi: 10.1093/nar/gks1078

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© The Author(s) 2012. Published by Oxford University Press.

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Figure 7. — The TNRC6 SD is sufficient for silencing. Mutations in the PAM2, CIM-1 and CIM-2 motifs were introduced in a minimal GW182 protein consisting of Caenorhabditis elegans ABD2 fused to the human TNRC6C SD region (ABD2-6C-SD). The PAM2 mutant carries a single amino acid substitution (F1389A) in the PAM2 motif. Mutations in the CIM-1 and CIM-2 motifs are shown in Supplementary Figure S7. (A and B) The silencing activity of the chimeric ABD2-6C-SD protein (wild-type or mutant) was tested in complementation assays as described in Figure 5. (C and D) The interactions of the ABD2-6C-SD protein (wild-type or mutant) with PABP and NOT1 were analyzed as described in Figure 2.