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. 2012 Dec 11;41(2):1319–1328. doi: 10.1093/nar/gks1222

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© The Author(s) 2012. Published by Oxford University Press.

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Figure 5. — PNRC2-dependent NMD substrates significantly overlap with SMG5-dependent NMD substrates. (A) Western blotting showing the specific siRNA-mediated downregulations. (B) Comparative analysis of normalized microarray data using total-cell RNAs purified from HeLa cells depleted of Upf1, SMG5, SMG7 or PNRC2. Transcripts upregulated or downregulated by at least 1.5-fold (left) or 2-fold (right) were chosen for analysis. (C) Venn diagrams showing the number of transcripts that were commonly upregulated by at least 1.5-fold upon downregulation of Upf1-interacting factors (SMG5, SMG7 or PNRC2) and upon downregulation of Upf1. The total number of commonly upregulated transcripts after siRNA downregulation is depicted in parentheses. (D) Venn diagrams showing the number of transcripts that were commonly upregulated by at least 2-fold upon downregulation of Upf1-interacting factors (SMG5, SMG7 or PNRC2) and upon downregulation of Upf1.