Table 2.
Thermal and thermodynamic parameters derived from DSC curves
| CCATCG*CAACC | CCATCG*CTACC | |||||||
|---|---|---|---|---|---|---|---|---|
| GGTAGCGTTGG |
GGTAGCGATGG |
|||||||
| −ΔH (kcal/mol) | −ΔS (eu) | −ΔG37 (kcal/mol) | Tm (°C) | −ΔH (kcal/mol) | −ΔS (eu) | −ΔG37 (kcal/mol) | Tm (°C) | |
| Controla | 79.1 | 214.9 | 12.4 | 64.5 | 75.0 | 203.9 | 11.8 | 63.0 |
| FABPa | 76.4 | 214.1 | 10.0 | 54.3 | 64.2 | 178.3 | 8.9 | 51.8 |
| FAFa | 65.7 | 180.7 | 9.7 | 55.4 | 58.6 | 160.1 | 8.9 | 53.4 |
| FAAFa | 39.7 | 106.1 | 6.8 | 42.7 | 33.9 | 88.8 | 6.4 | 39.7 |
| ΔΔH (kcal/mol)b | ΔΔS (eu)c | ΔΔG37 (kcal/mol)d | ΔTm (°C)e | ΔΔH (kcal/mol)b | ΔΔS (eu)c | ΔΔG37 (kcal/mol)d | ΔTm (°C)e | |
| FABPa | 2.7 | 0.8 | 2.4 | −10.2 | 10.8 | 25.6 | 2.9 | −11.2 |
| FAFa | 13.4 | 34.2 | 2.7 | −9.1 | 16.4 | 43.8 | 2.9 | −9.6 |
| FAAFa | 39.4 | 108.8 | 5.6 | −21.8 | 41.1 | 115.1 | 5.4 | −23.3 |
The average standard deviations for ΔG, ΔH, and Tm are ±0.2, ±2.0, and ±0.4, respectively.
aThe results were calculated from integration of the DSC curve directly. ΔG and ΔH represent the heat absorbed during duplex melting at 0.12 mM.
bΔΔH = ΔH (modified duplex) − ΔH (control duplex).
cΔΔS = ΔS (modified duplex) − ΔS (control duplex).
dΔΔG = ΔG (modified duplex) − ΔG (control duplex).
eΔTm = Tm (modified duplex) − Tm (control duplex).