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. 2012 Nov 26;41(2):775–789. doi: 10.1093/nar/gks1185

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Published by Oxford University Press 2012.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 2. — Tf1 insertion increased the expression of ssa1. (A) The levels of ssa1 mRNA from cells lacking Tf1 and from cells with Tf1 upstream of ssa1 was measured on RNA blots. The amount of ssa1 mRNA relative to actin mRNA is shown in the histogram. (B) The transcription start site of ssa1 mRNA from cells with and without the Tf1 insertion at ssa1 was mapped by 5′ RACE and sequencing of the products. (C) The levels of ssa1 mRNA from cells without Tf1 and from all the 14 strains with Tf1 insertions were measured on an RNA blot. The histogram shows for each insertion strain the change in the expression of ssa1 mRNA caused by the insertion of Tf1.