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HPLC chromatograms of a CoA activation assay with nondesalted cell extracts of the skt mutant Pseudomonas sp. strain G12 containing DHOCTO (XX in Fig. 1) as the substrate incubated for 0 min (A) and 160 min (B). The analysis wavelengths were 245 nm (gray) and 260 nm (black). P8 was identified as the CoA ester of DHOCTO (IX) and was hydrolyzed completely after treatment with NaOH (C).
