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DEAD-box RNA helicases are dispensable at 37°C. The B. subtilis wild-type strain 168 and the isogenic ΔcshA, ΔcshB, ΔdeaD, and ΔyfmL mutant strains were grown at 37°C in LB medium. In addition, growth of a quadruple mutant devoid of all four RNA helicases (Δ4) and of a strain with ectopic expression of cshA was recorded.
