Fig 4.

CshA is the most abundant RNA helicase in B. subtilis. Crude extracts were isolated from the B. subtilis strains GP1010 (cshA-FLAG), GP1011 (cshB-FLAG), GP1068 (deaD-FLAG), and GP1066 (yfmL-FLAG) grown in LB medium at 20°C. Fifteen μg of protein of the crude extract of each culture was loaded onto a 12.5% SDS-polyacrylamide gel. After electrophoresis and blotting onto a polyvinylidene difluoride (PVDF) membrane, the FLAG tag was detected using rabbit polyclonal antibodies. All cultures were harvested in the exponential (A), transition (B), or late exponential phase (C).