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Effects of the deletion of the RNA helicase cshA on the ysbA (A) and frlB (B) mRNAs. Total RNA was isolated from the B. subtilis wild-type strain 168 and the isogenic cshA mutant GP1083 grown in minimal medium supplemented with 0.5% glucose. Five μg of total RNA was loaded per lane. A schematic illustration of the operon structure is given below each Northern blot.
