Table 1.
Bacillus subtilis strains used in this study
| Strain | Genotype | Source or referencea |
|---|---|---|
| 168 | trpC2 | Laboratory collection |
| GP899 | trpC2 Δnfo::aphA3 | 27 |
| GP1010 | trpC2 cshA-FLAG spc | 5 |
| GP1011 | trpC2 cshB-FLAG spc | 5 |
| GP1026 | trpC2 cshA-Strep aphA3 | 5 |
| GP1035 | trpC2 ΔcshA::aphA3 | This study |
| GP1051 | trpC2 ΔcshB::cat | This study |
| GP1052 | trpC2 ΔdeaD::tet | This study |
| GP1053 | trpC2 ΔyfmL::mls | This study |
| GP1063 | trpC2 ΔcshA::aphA3 ΔcshB::cm ΔdeaD::tet ΔyfmL::mls | This study |
| GP1066 | trpC2 yfmL-FLAG spc | This study |
| GP1068 | trpC2 deaD-FLAG spc | This study |
| GP1083 | trpC2 ΔcshA::cat | This study |
| GP1084 | trpC2 ΔcshA::cat lacA::cshA aphA3 | pGP1886→GP1083 |
| GP1086 | trpC2 ΔcshB::cat lacA::cshB aphA3 | pGP1857→GP1051 |
| GP1087 | trpC2 ΔyfmL::mls lacA::yfmL aphA3 | pGP1858→GP1053 |
| GP1095 | trpC2 cshA-Strep aphA3 rplA-FLAG spc | pGP1895→GP1026 |
a
Arrows indicate construction by transformation.