Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli BL21(DE3)pLysS | For overproduction of proteins | Novagen |
| Salmonella enterica | ||
| SJW1103 | Wild type for motility and chemotaxis | 30 |
| SJW1368 | ΔcheW-flhD | 45 |
| MKM40 | ΔfliJ | 25 |
| MMB017 | flhB(P28T) | 11 |
| MMHI001 | ΔfliH-fliI | 32 |
| MMHI0117 | ΔfliH-fliI flhB(P28T) | 11 |
| MMHIJ0117 | ΔfliH-fliI-fliJ flhB(P28T) | 27 |
| Plasmids | ||
| pET15b | Expression vector | Novagen |
| pGEX6p-1 | Expression vector | GE Healthcare |
| pTrc99A | Expression vector | GE Healthcare |
| pTrc99AFF4 | Expression vector | 31 |
| pMM104 | pET19b/His-FlhAc | 13 |
| pMM404 | pTrc99AFF4/FliJ | 10 |
| pMMIJ001 | pET15b/His-FliJ | 33 |
| pMMJ1001 | pGEX6p-1/GST-FliJ | 25 |
| pTIJ007 | pET15b/His-FliJ(F72A) | This study |
| pTIJ008 | pET15b/His-FliJ(L76A) | This study |
| pTIJ105 | pTrc99AFF4/FliJ(F72A) | This study |
| pTIJ106 | pTrc99AFF4/FliJ(L76A) | This study |
| pTIJ201 | pGEX6p-1/GST-FliJ(Q38A) | This study |
| pTIJ202 | pGEX6p-1/GST-FliJ(L42A) | This study |
| pTIJ203 | pGEX6p-1/GST-FliJ(Y45A) | This study |
| pTIJ204 | pGEX6p-1/GST-FliJ(Y49A) | This study |
| pTIJ205 | pGEX6p-1/GST-FliJ(F72A) | This study |
| pTIJ206 | pGEX6p-1/GST-FliJ(L76A) | This study |
| pTIJ207 | pGEX6p-1/GST-FliJ(A79S) | This study |
| pTIJ208 | pGEX6p-1/GST-FliJ(H83A) | This study |