Fig 2.

ArcS Western blot analysis of localization and production. Membrane (M) and cytosolic (C) fractions of S. oneidensis MR 1 cells bearing a copy of plasmid-encoded His₆-ArcS (ΔarcS + pBAD33-HisA-ArcS, left lanes), lacking ArcS (ΔarcS, middle lanes), or with a chromosomally encoded His₆-ArcS version (KI-His6-arcS; right lanes) were analyzed for the presence of ArcS by SDS-PAGE using either Coomassie stain (upper panel) or His₆-specific antibodies (lower panel). The exposure time for chemiluminescence development is given in minutes.