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In vitro studies of phosphotransfer from ArcA to ArcS. (A) Autoradiographic analysis of phosphotransfer between the indicated variants of ArcSaa694-1236, GST-HptA, and ArcA. Phosphorylated ArcA was incubated for 1 min with the indicated components, and the samples were then separated by SDS-PAGE and analyzed for their radioactive signal intensities. (B) Corresponding signal intensities were calculated using ImageJ and are given as normalized numbers, setting each lane as 100%.
