Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotypea | Source or reference |
|---|---|---|
| Escherichia coli strains | ||
| DH5αλpir | recA1 gyrA Δ(lacIZYA-argF) (ϕ80d lac [lacZ] M15) pir RK6 | 50 |
| WM3064 | thrB1004 pro thi rpsL hsdS lacZ ΔM15 RP4-1360 Δ(araBAD) 567ΔdapA 1341::[erm pir(wt)] | W. Metcalf, University of Illinois, Urbana-Champaign |
| Shewanella oneidensis MR-1 strains | ||
| S79 | Wild type | 51 |
| ΔarcS mutant | ΔSO_0577 (arcS) | 23 |
| KI-His6-arcS | Chromosomal replacement of native arcS by a mutated version of arcS encoding an N-terminal His6 tag | This study |
| KI-His6-arcS PcsgAB-lux | (KI-His6-arcS); csgAB::PcsgAB-lux | This study |
| KI-arcS-HKH731A | Chromosomal replacement of native arcS by a mutated version encoding arcS (H731A) | This study |
| KI-arcS-HKH731A PcsgAB-lux | arcS (H731A); csgAB::PcsgAB-lux | This study |
| KI-arcS-RecID1017N | Chromosomal replacement of native arcS by a mutated version encoding arcS (D1017N) | This study |
| KI-arcS-RecID1017N PcsgAB-lux | arcS (D1017N); csgAB::PcsgAB-lux | This study |
| KI-arcS-RecIID1162N | Chromosomal replacement of native arcS by a mutated version encoding arcS (D1162N) | This study |
| KI-arcS-RecIID1162N PcsgAB-lux | arcS (D1162N); csgAB::PcsgAB-lux | This study |
| KI-arcS-RecI+IID1017/1162N | Chromosomal replacement of native arcS by a mutated version encoding arcS (D1017N; D1162N) | This study |
| KI-arcS-RecI+IID1017/1162N PcsgAB-lux | arcS (D1017N; D1162N); csgAB::PcsgAB-lux | This study |
| KI-arcS-PASI464-573 | Chromosomal replacement of native arcS by a mutated version encoding arcS (ΔPAS464-573) | This study |
| KI-arcS-PASI464-573 PcsgAB-lux | arcS (ΔPAS464-573); csgAB::PcsgAB-lux | This study |
| KI-arcS-PASII594-698 PcsgAB-lux | Chromosomal replacement of native arcS by a mutated version encoding arcS (ΔPAS594-698) | This study |
| KI-arcS-PASII594-698 | arcS (ΔPAS594-698); csgAB::PcsgAB-lux | This study |
| ΔarcA mutant | ΔSO_3988 (arcA) | 23 |
| ΔcsgAB mutant | ΔSO_0865-SO_0866 (csgAB) | This study |
| Plasmids | ||
| pGP704Sac28Km | mobRP4 ori-R6KsacB, suicide plasmid for generating in-frame deletions or integrations; Kmr | Chengyen Wu, unpublished |
| pGP704Sac28Km-KI-arcS-HKH731A | arcSH731A in pGP704Sac28Km | This study |
| pGP704Sac28Km-KI-arcS-RecID1017N | arcSD1017N in pGP704Sac28Km | This study |
| pGP704Sac28Km-KI-arcS-RecIID1162N | arcSD1126N in pGP704Sac28Km | This study |
| pGP704Sac28Km-KI-arcS-RecI+IID1017/1162N | arcSD1017N/D1126N in pGP704Sac28Km | This study |
| pBBR1-MCS5-TT-RBS-lux | oriT mobRK2 oriR lacZα, luxCDABE followed by terminators lambda T0 rrnB1 T1, reporter system for plasmid-based transcriptional fusions; Gmr | 38 |
| pBBR1-MCS5-TT-RBS-lux-PcsgAB | PcsgAB promoter fragment in pBBR1-MCS5-TT-RBS-lux | |
| pNPTS138-R6KT | oriT ori-R6K sacB, suicide plasmid for generating in-frame deletions or integrations; Kmr | 23 |
| pNPTS138R6KT-Lux-PcsgAB | PcsgAB-lux fragment from pBBR1-MCS5-TT-RBS-lux-PcsgAB in pNPTS138-R6KT | This study |
| pNPTS138-R6KT-KI-His6-arcS | ATG-His6-arcS fragment in pNPTS138-R6KT | This study |
| pNPTS138-R6KT-KI-arcS-PASI464-573 | arcSΔPAS-I (Δ aa 464–573) in pNPTS138-R6KT | This study |
| pNPTS138-R6KT-KI-arcS-PASII594-698 | arcSΔPAS-II (Δ aa 594–698) in pNPTS138-R6KT | This study |
| pNPTS138-R6KT-ΔcsgAB | ΔcsgAB fragment in pNPTS138-R6KT | This study |
| pBAD-HisA-arcS-694 | arcS (aa 694–1236) in pBAD-HisA | 23 |
| pBAD-HisA-arcS-694H731A | arcSH731A (aa 694–1236) in pBAD-HisA | This study |
| pBAD-HisA-arcS-694H731A/D1017N | arcSH731A/D1017N (aa 694–1236) in pBAD-HisA | This study |
| pBAD-HisA-arcS-694 H731A/D1162N | arcS H731A/D1162N (aa 694–1236) in pBAD-HisA | This study |
| pBAD-HisA-arcS-694 | arcSH731A/D1017/1162N (aa 694–1236) in pBAD-HisA | This study |
| pBAD-HisA-arcA | arcA in pBAD-HisA | 23 |
| pGEX4T-1 | ori pBR322 lacI gst, vector for overproduction of GST-fused proteins; Apr | GE Healthcare |
| pGEX4T-1-hptA | hptA in pGEX4T-1 | 23 |
| pBAD33-HisA | ori-p15A araC PBAD, vector for arabinose-inducible production of His-tagged proteins; Cmr | N. Hess, unpublished |
| pBAD33-HisA-arcS-HKH731A | arcSH731A in pBAD33-HisA | This study |
| pBAD33-HisA-arcS-RecID1017N | arcSD1017N in pBAD33-HisA | This study |
| pBAD33-HisA-arcS-RecIID1162N | arcSD1162N in pBAD33-HisA | This study |
| pBAD33-HisA-arcS-RecI+IID1017/1162N | arcSD1017/1162N in pBAD33-HisA | This study |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Gmr, gentamicin resistance; Kmr, kanamycin resistance.