Fig 5.

PmucR activity is induced in B. melitensis growing in 2YT medium containing 400 mM NaCl. (A) Growth curve of the reporter [B. melitensis WT expressing the transcriptional fusion PmucRgfp(ASV)] and the control [the WT harboring the plasmid pBBR-gfp(ASV)] strains in both 2YT medium (85.5 mM NaCl) and 2YT medium containing 400 mM NaCl. (B) Fluorescence intensity measured by flow cytometry (5 × 10⁴ events acquired) of WT B. melitensis expressing the transcriptional fusion PmucRgfp(ASV) at the end of preculture in 2YT medium. The WT harboring the plasmid pBBR-gfp(ASV) was used as a negative control. (C to H) Fluorescence intensities measured in the reporter strains growing in unsupplemented 2YT medium or 2YT medium supplemented with 314.5 mM NaCl at 2 h (C), 8 h (D), 12 h (E), 24 h (F), 48 h (G), and 72 h (H) postinoculation. The insets are phase-contrast and corresponding fluorescence images of the major morphotype of the reporter strain in 2YT medium containing 400 mM NaCl at the indicated times. Scale bar, 2 μm.