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. 2013 Feb;195(3):596–605. doi: 10.1128/JB.02005-12

Fig 4.

Fig 4

Capsule assembly in wild-type and csaB mutant B. cereus G9241. (A) B. cereus G9241 wild-type and csaB mutant were grown at 30°C for 5 h in BHI broth with 50% fetal bovine serum (BHI-FBS). Mutant bacilli harbored no plasmid, the pLM4 vector, or the complementing plasmid pcsaB. Capsule formation was detected by microscopy of india ink-stained samples. Data are representative of three independent experimental determinations. (B) B. cereus G9241 mutants lacking capsular polysaccharide operons responsible for BPS (ΔbpsX-H), hyaluronic acid (HA; ΔhasACB), or BPS and HA production (ΔhasACB ΔbpsAB) were transduced with the csaB::Ω-Sp allele. Capsule formation was detected by microscopy of india ink-stained samples. Data are representative of three independent experimental determinations.