Fig 5.

Expression of B. cereus G9241 S-layer proteins and S-layer-associated proteins under capsule-inducing conditions. B. cereus G9241 was grown under capsule-inducing (BHI-FBS) or noninducing (BHI) conditions to (A) exponential (A₆₀₀ = 1) or (B) late exponential/stationary phase (A₆₀₀ = 4). Cultures were centrifuged to sediment vegetative bacilli, and the extracellular medium was removed. Proteins were precipitated with TCA and analyzed by immunoblotting with rabbit antisera raised against purified recombinant Sap (αSap), EA1 (αEA1), BslA (αBslA), BslO (αBslO), and membrane protein PrsA (αPrsA). The abundance of S-layer proteins and S-layer-associated proteins in the envelopes of B. cereus G9241 wild-type, csaB, csaB(pcsaB), and ΔhasACB ΔbpsAB strains was compared. Data are representative of three independent experimental determinations.