Fig 5.

Potential role for quorum sensing in C. difficile biofilm formation. (A) Biofilm formation by WT R20291, putative quorum-sensing gene luxS mutant (luxS), and complemented strains (luxS-C) as measured by CV staining. (B) Confocal microscopy analysis of WT, luxS, and complemented strain luxS-C. The results are presented in log scale, and the error bars represent the standard deviations (P < 0.05). The data from biofilm assays are representative of at least three independent experiments performed in triplicates.