Table 1.

Oligonucleotide primers and probes used in this study

Name	Sequence (5′ to 3′)a	Siteb	Strand	Positionc
cagBA-IGR-5′P	tttgggtaccTTTTTAATCGTCTCAGGTTCA	KpnI	+	579301–579321
cagBA-IGR-3′P	ctagccatggGACTATCGGTATCTTATTGGTATCA	NcoI	−	579869–579892
5′-ureB sRNA(+)-5′P	CTTGCcAtgGCTGTAGGGATTTGTTGGGGTG	NcoI	+	76785–76815
5′-ureB sRNA(+)-3′P	CTGAGAGAAGGCATGAGCCA		−	77067–77086
HP0092-T1-5′P-rev	AGTTTAATTACCAGTGGATA		+	98324–98343
HP0092-T1-5′P	TATCCACTGGTAATTAAACT		−	98324–98343
HP0092-T1-3′P	ATTTcTGCaGATCCGCCTTATTTCCTCTCT	PstI	+	98206–98235
HP0547-5′P(548-571)	AGGCTCATTTCTTATTTCTTGTTC		+	579635–579658
HP0547-3′P(1785-1806)	TGTTTCTCCTTACTATACCTAG		−	579899–579920
P-PcagA	CTAGGTATAGTAAGGAGAAACA		+	579899–579920
HP0072-5′P(1111-1133)	CTACAGGCGATAAAGTGAGATTG		−	77168–77190
HP0072-3′P(1902-1923)	TAATATCAGGAGCGTGTCCGCC		+	76378–76399
HP0072-5′P(1266-1290)	CTAATCATCACTAACGCTTTAATCG		−	77011–77035
HP0072-3′P(2653-1673)	CAATGTGAGCGGTAGTGTCGT		+	75628–75648
HP0072-5′P(1215-1234)	CTGAGAGAAGGCATGAGCCA		−	77067–77086
HP0072-3′P(1486-1456)	CTTGCAAAAGCTGTAGGGATTTGTTGGGGTG		−	76785–76815
HP0072-5′P(2159-2182)	TATGGGTCGTGTGGGTGAAGTTAT		−	76119–76142
HP0072-3′P(2653-2673)	CAATGTGAGCGGTAGTGTCGT		+	75628–75648
Mut-YUAN-up	CAAAGACATGCAAGATGGCGgaAcAAACAATCTTAGCGTAGGTC		−	76891–76934
Mut-YUAN-dn	GACCTACGCTAAGATTGTTTgTtcCGCCATCTTGCATGTCTTTG		+	76891–76934
5′-ureB 2-1S	CTAATCATCACTAACGCTTTAATCGTGGATTACACCGGTATTTATAAAGCGGATA		−	76981–77035
5′-ureB 2-1AS	CTAATCATCACTAACGCTTTAATCGTGGATTACACCGGTATTTATAAAGCGGATA		+	76981–77035

^aSequences in uppercase letters are derived from the genome sequences of H. pylori 26695 (30). Sequences introduced for cloning or mutation purposes are given in lowercase letters, and restriction recognition sites are underlined.

^bRestriction recognition sites.

^cNucleotide positions refer to the genome sequence of H. pylori 26695 (30).