Fig 3.

Minimum requirements for primary replication. The total numbers of plaques in each experiment were counted when BSR cells were first transfected with 6 expression plasmids, VP1, VP3, VP4, VP6, VP7, and NS2 (Core + NS2), or with 5 expression plasmids, VP1, VP3, VP4, VP6, and NS2 (Subcore + NS2), or 4 expression plasmids, VP1, VP4, VP6, and NS2 (VP1, 4, 6 + NS2). The transfected cells were transfected a second time with all 10 uncapped transcripts (S1 to S10). The recovery of BTV was compared with that for the control (open column), in which BSR cells were transfected only once with the 10 capped transcripts (S1 to S10). The recovery of virus was shown as a fold of that for the control (mean ± SD).