Fig 6.

Neuraminidase treatment but not inhibition of ganglioside synthesis decreases cell surface binding of rotavirus strains RRV and TFR-41. MA104 cells were transfected with the indicated siRNAs, and 72 h posttransfection, the cell monolayers were incubated or not with 40 mU of neuraminidase for 1 h at 37°C. After this time, cells were blocked with BSA, and purified rotavirus TLPs were added for 1 h at 4°C, as described in Materials and Methods. Virus bound to cells was determined by an ELISA. Data are expressed as the percentage of virus bound to the cells relative to virus detected in siIrr control treatment cells. The means and standard errors of results from three independent experiments are shown. Statistical analysis was done using a Student t test, and statistically significant values are shown as follows: ***, P < 0.001; **, P < 0.01; *, P < 0.05.