Fig 7.

Preincubation with gangliosides affects rotavirus infectivity. Four different rotavirus strains (RRV, TFR-41, Wa, and UK) were preincubated with the indicated gangliosides (GA1, GM1a, GM2, GD1b, GD1a, GM3, GD3) in three different concentrations (1.5 [black bars], 12.5 [white bars], and 50 [striped bars] μg/ml). After preincubation, the virus-ganglioside mixture was used to infect MA104 cells. Fourteen hours postinfection, cells were fixed and stained as described in Materials and Methods. The results are expressed as the percentages of infectivity relative to control (preincubation with 0 μg/ml) treatment. The means and standard errors from results of at least three independent experiments are shown. Statistical analysis was done using a Student one-way ANOVA test and a Tukey posttest, and statistically significant values for highest ganglioside concentration are shown as follows: ***, P < 0.001; **, P < 0.01; *, P < 0.05.