Fig 2.

E1A-13S colocalizes with endogenous PML in infected cells. Human hepatocytes (HepaRG) were infected with wild-type (H5pg4100) or mutant (H5dl347, H5dl348, H5pm4149, and H5pm4150) viruses at a multiplicity of 50 FFU/cell and fixed with 4% PFA at 24 h p.i. The cells were extracted with cytoskeleton buffer prior to fixation and double labeled with MAb M-58 (α-E1A) and PAb NB 100-59787 (α-PML). Detection of primary Abs and nuclear staining were performed as for Fig. 1. Representative α-E1A (red) (c, g, k, o, s, and w) and α-PML (green) (b, f, j, n, r, and v) staining patterns of at least 50 analyzed cells are shown. Overlays of the single images (merge) are shown in panels d, h, l, p, t, and x. Magnification, ×7,600.