Fig 5.

N and L RNA transcript levels of recombinant VSVs. The amounts of N-protein mRNA (A) or L-protein mRNA (B) generated by VSV-12′GFP and three other recombinant VSVs (rVSV, VSV-G/GFP, and VSV-1′GFP) were compared using quantitative RT-PCR and are expressed as transcript copy number per μl of total RNA. Genomic RNA was distinguished from mRNA by utilizing a probe specific to an intergenic region of the VSV genome; genomic values were subtracted from total RNA to yield mRNA values. Cultures of U-87 cells were inoculated (0.1 MOI) in triplicate for each condition, and total RNA was harvested 12 h postinfection. The number above each bar indicates the genomic position from which the transcript arises, and the error bars represent the standard errors of the means. All mean transcript values were significantly different (ANOVA; P < 0.01) from each other except those for 1′GFP and 12′GFP in the N mRNA panel.