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Immunoprecipitation of hDaxx and ATRX from control and pp71 cell samples. Extracts from uninduced and induced (100 ng/ml doxycycline for 24 h) control and pp71 cells were used for immunoprecipitation with control nonspecific or anti-ATRX and anti-hDaxx rabbit antibodies, as indicated. Samples of the clarified extract and the immune precipitates were analyzed by Western blotting for ATRX, hDaxx, and Myc-tagged pp71 using MAbs 39F, Daxx-01, and 9E10, respectively.
