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Only the 105-amino-acid form of capsid protein is detected in WNV-infected cells. A549 and HEK293T cells were infected with WNV at an MOI of 5, and at the indicated time points, cell lysates were prepared and analyzed by immunoblotting with antibodies to capsid and GAPDH (loading control). As indicated, cells were transfected with plasmid pCMV5-mCap, which encodes the 105-amino-acid (105 aa) form of capsid, or pCMV5-aCap, which encodes the 123-amino-acid (123 aa) form of capsid.
