Fig 5.

WNV capsid inhibits Fas-mediated apoptosis in HEL/18 cells. HEL/18 cells were transduced with lentiviruses encoding AcGFP alone (AcGFP) or AcGFP and WNV capsid protein (Capsid) at a multiplicity of transduction of 3. At approximately 48 h postransduction, cells were treated with or without anti-Fas (Fas). Samples were then processed for intracellular staining with antibodies to active caspase-3, followed by staining with anti-rabbit Alexa Fluor 647. (A) Samples were then analyzed via FACS, and histogram overlays were constructed from a representative experiment in which capsid-expressing cells (unfilled histograms) were compared to AcGFP-only-expressing cells (gray-filled histograms). (B) Results averaged from 3 independent experiments are shown. Standard error bars are indicated. Statistical analyses (paired t test) were performed, and statistically significant differences are indicated (*, P ≤ 0.05). (C) Histograms display the levels of AcGFP expression and thus transduction in experimental samples. (D) Immunoblot data show that transduction of cells with lentiviruses encoding WNV capsid results in robust expression.