Fig 1.

Expression of Notch and ORF2 in TG. TG were collected from calves that were mock infected, latently infected (at least 60 days postinfection), or latently infected and treated with DEX at the indicated times (hours [H]) after DEX treatment to induce reactivation from latency. Thin sections were cut and subjected to immunohistochemistry analysis with antibodies that specifically recognize Notch3 (A), Notch1 (B), or ORF2 (C) as described in Materials and Methods. Biotinylated goat anti-rabbit IgG was used as a secondary antibody. Thin sections from mock-infected calves were used as a negative control. Arrows indicate positive neurons.