Table 1.
Summary of the Notch-inhibiting functions of ORF2 and a panel of mutant ORF2 constructsa
| Construct | Inhibition of: |
||||
|---|---|---|---|---|---|
| Activation of BHV-1 promoters | Activation of productive BHV-1 infection | Neurite formation | HES5 activation | Notch3 degradation | |
| ORF2 | +++ | +++ | +++ | +++ | +++ |
| ORF2-95 | − | − | − | +++ | +++ |
| ORF2-134 | − | − | − | +++ | +++ |
| ORF2-240 | − | − | − | +++ | +++ |
| ORF2-271 | − | − | − | +++ | +++ |
| ORF2-310 | +++ | +++ | +++ | +++ | +++ |
| ORF2-333 | +++ | +++ | +++ | +++ | +++ |
| ORF2-469 | +++ | +++ | +++ | +++ | +++ |
| ORF2-529 | +++ | +++ | +++ | +++ | +++ |
| ORF2-NLS | − | − | − | − | − |
| ORF2-P | +++ | +++ | +++ | − | +++ |
| ORF2-AP | +++ | +++ | +++ | − | +++ |
a
Studies describing the effects of ORF2 on the activation of BHV-1 promoters and productive infection were previously published (26, 44). The abilities of ORF2 to inhibit neurite formation, HES5 promoter activation, and Notch3 degradation are presented in this study. +++, inhibition; −, lack of inhibition.