Fig 4.

MeV infection induces early extracellular release of hsp70 from viable neuronal cells. Mouse neuroblastoma (N2a) cells were infected with Ed MeV at an MOI of 1.0 and monitored for 60 h p.i. The N2a cells were stably transfected to constitutively express hsp70 (N2a-HSP) or contained empty vector (N2a-V). Virus-induced cell death was not observed, based upon lack of a significant difference in LDH release between infected and uninfected cells (−). Extracellular hsp70 levels were measured by ELISA. Results reflect levels measured over infected (Inf) and uninfected (Uninf) cells at discrete postinfection intervals. The shaded region represents background measured over uninfected cells, and asterisks denote significant increases over background (ANOVA; P < 0.05). Detergent-lysed cells served as positive controls for the LDH and hsp70 analyses.