Fig 2.
Cytoplasmic localization of EGFP-NEPΔNES1 is sensitive to the nuclear export inhibitor LMB. (A) Cytoplasmic localization of EGFP-NEPΔNES1 is inhibited following treatment with LMB in MDCK cells. (B) Cytoplasmic localization of EGFP-NEPΔNES1 in HeLa cells is inhibited following treatment with LMB. (C) Subcellular localization of the negative control EGFP is not sensitive to LMB. MDCK or HeLa cells were transiently transfected with the EGFP-NEPΔNES1 plasmid or the control vector pEGFP-C1 and treated (+LMB) or not treated (−LMB) with 11 nM LMB for 3 h at 21 h posttransfection. Cells were then fixed, permeabilized, and stained with Hoechst 33258 dye. Bars, 10 μm.