Fig 1.

MxA is necessary for control of human seasonal influenza virus replication in IFN-α-stimulated LLC-MK₂ cells. (A) Effect of IFN-α administration on MxA mRNA expression 24 h after treatment. Bars represent an average of three replicate cultures (mean ± standard error). (B) Effect of IFN-α administration on A/Memphis/7/01 virus replication. Values represent the log₁₀ copies of matrix RNA/ml of culture supernatant. Bars are an average of three replicate cultures (mean ± standard error). (C) Knockdown of rhesus macaque MxA. Cell cultures were transfected with siRNAs against MxA (siMxA) or with AllStar negative-control siRNA (siNeg), and 24 h later, IFN-α was added. Bars represent the average of two experiments run in triplicate (mean ± standard error). (D) Immunofluorescence staining of cytospin slides of cells transfected with siMxA or siNeg and inoculated with IFN-α for MxA (red) and DAPI (blue). (E) Infectious influenza virus titers (TCID₅₀/ml) in supernatant of cultures transfected with siMxA or siNeg. Six hours later, IFN-α was added, and 24 h after that, the cells were infected with A/Memphis/7/01 and compared to control cultures infected with A/Memphis/7/01 alone or treated with IFN-α and then infected with A/Memphis/7/01. Bars represent the average of four experiments run in triplicate (mean ± standard error). The P value was generated using an ANOVA, and the results of Tukey's post hoc pairwise comparisons are shown only if differences were significant.