Fig 3.

A/Memphis/7/01 replication is inhibited in Vero cells stably transfected with human MxA. (A) MxA mRNA levels were assessed by TAQman PCR in untreated MxA-transfected Vero cells (MxA⁺; VA9) and empty-plasmid-transfected control Vero cells (MxA⁻; VN36) treated with IFN-α. The levels of MxA mRNA in these cultures relative to those in untreated VN36 cell cultures are shown. Bars represent the average of three samples (mean ± standard error). (B) Immunofluorescence was performed on cytospins of VA9 and VN36 cells for MxA (red) and nuclei (blue). (C) VA9 and VN36 cells were infected with A/Memphis/7/01, and the levels of nucleoprotein (NP) gRNA were assessed 8 h later. (D) VA9 and VN36 cells were infected with A/Memphis/7/01, and the levels of matrix gRNA were assessed 8 h later. Values presented are negative-strand gRNA copies per cell. Bars represent data from two experiments run in triplicate (mean ± standard error). (P values were generated with a two-tailed Student t test.)