ANDV Gn protein colocalizes with LAMP2 and mTOR in infected cells. HMVEC-L cells were infected with ANDV for 3 days and stained with antibodies recognizing endogenous LAMP2, mTOR, and viral protein Gn. Nuclei are outlined in white. Note the doughnut-like mTOR- and LAMP2-positive vesicles in mock-transfected cells (mock, inset, yellow arrow) and ANDV-infected cells (inset, yellow arrow). Gn localizes within LAMP2- and mTOR-positive vesicles (inset, yellow arrow) and occasionally exhibits doughnut-like structures (inset, white arrowhead). Scale bar, 10 μm.