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. 2013 Jan;87(2):1232–1241. doi: 10.1128/JVI.02441-12

Table 2.

Comparison of experimental and calculated sedimentation coefficients (s20,w) for different Rep68 oligomers

Oligomer Mass (kDa)
s20,w (S)
Sequence Experimentala Experimentala Calculated from modelb
Monomer 60.8 61.4 3.7 3.4
Dimer 121.6 114 5.4 5.5
Trimer 182.4 215c 8.7c 7
Tetramer 243.2 8.3
Hexamer 364.8 NDd ND 11.1
Heptamer 425.6 412c 13.3c 12.5
Octamer 486.4 13.6
a

Mass and sedimentation coefficients calculated from the corresponding peak position of the sedimentation profiles and f/f0 values determined with the SEDFIT program.

b

Sedimentation coefficients calculated from atomic models using the program UltraScan-SOMO.

c

Calculated from broad peaks that do resolve into two or more peaks with these data.

d

ND, not determined.