Table 2.
Summary of protein phosphorylation in HearNPV BV and ODV identified by IMAC
| Protein | Phosphopeptide sequencea | Phosphorylated site | Site determination |
Motif (eukaryotic kinase)b | |
|---|---|---|---|---|---|
| BV | ODV | ||||
| POLH | MpYTRYpSYpSPpTLGK | Y2 | No | Yes | |
| S6 | No | Yes | PKA (R.p[ST]) | ||
| S8 | Yes | No | |||
| T10 | No | Yes | |||
| NLDpSLDKYLVAEDPFLGPGK | S50 | No | Yes | ||
| NQKLpTLFK | T71 | No | Yes | Aurora ([RK].p[ST][ILV]) | |
| IKEFAPDAPLYTGPApY | Y246 | No | Yes | ||
| P78/83 | NQLNQRLpSNAQTQQISAK | S311 | No | Yes | PKA (R.p[ST]) |
| E18 | NNPFVNpTPQRpTMM | T75 | No | Yes | CDK2 (p[ST]P.[KR]), ERK ([VX]p[ST]P), CDK1 (p[ST]P.[KR]) |
| T79 | No | Yes | |||
| EC27 | TVpTEIINADEK | T15 | No | Yes | CAMK2 (R . . p[ST]) |
| PIF5 | RQpSVKpTNFPSTNTR | S118 | No | Yes | PKA (R.p[ST], R[RK].p[ST]), CAMK2 (R . . p[ST], R . . p[ST]V), Aurora ([RK].p[ST][ILV]), Aurora-A ([RKN]R.[ST][MLVI]) |
| T121 | No | Yes | CK1 (S . . p[ST]) | ||
| LEF6 | STDRIpSEIGDWCR | S140 | No | Yes | PKA (R.p[ST]), CK1 ([ST] … pS) |
| HA44 | SLGLMpSR | S23 | No | Yes | |
| RPPpSPMNVSESTTIVPQSQR | S138 | Yes | Yes | CAMK2 (R . . p[ST]), ERK (P.p[ST]P) | |
| VLF1 | HLpSVDDLNTLIK | S221 | No | Yes | CAMK2 (R . . p[ST], R . .p[ST]V) |
| LRpSTIGLK | S236 | No | Yes | ||
| GP41 | QLQESSpSDAWTNK | S25 | No | Yes | |
| FDpSDENLIK | S82 | No | Yes | PKA (KR . . p[ST]), CAMK2 (R . . p[ST]), PKD ([LVI].[RK] . . p[ST]), CHK1 ([MILV].[RK] . . p[ST]) | |
| RPpSLFQGATFLNAPSSNGSNVEQNR | S269 | No | Yes | PKA (R.p[ST]), Aurora ([RK].p[ST][ILV]), Aurora-A ([RKN]R.p[ST][MLVI]) | |
| VP39 | ITpSEGLLASVR | S225 | No | Yes | CAMK2 (R . . p[ST]) |
| E25 | PLpTYpSEIIDEGNR | T80 | No | Yes | CK2 (p[ST] . . E), CAMK2 (R . . p[ST]) |
| S82 | No | Yes | NEK6 (L . . p[ST]) | ||
| TVGANCVFMGTISEPSQTSTLNQQQQQQQSAGSpSLPTTANR | S124 | No | Yes | CK1 ([ST] … pS) | |
| VpTANFDIK | T133 | No | Yes | PKA (R.p[ST]) | |
| C42 | QPpSRGNLLNLFR | S98 | No | Yes | CAMK2 (R . . p[ST]) |
| VP80 | NEpSETLPASTSIR | S121 | No | Yes | CAMK2 (R . . p[ST]) |
| TRpSPTTSNEK | S345 | No | Yes | GSK3 (pS . . S), CAMK2 (R . . p[ST]); AKT (R[RST].p[ST].[ST]) | |
| BRO-C | RHpSNAGDVYSAQQVVHAMK | S435 | No | Yes | PKA (R.p[ST], R[RK].p[ST]), CAMK2 (R . . p[ST]) |
| PEP | CWpSDFK | S55 | Yes | No | PKA (KR . . p[ST]), CAMK2 (R . . p[ST]) |
| HOAR | QEPVDMSDVECLNLPLpSPAR | S698 | No | Yes | CDK2 (p[ST]P.[KR]), ERK (P.p[ST]P), CDK1 (p[ST]P.[KR]), NEK6 (L . . p[ST]) |
| HA39 | ISSLSLRApSV | S193 | No | Yes | PKA (R.p[ST]) |
| HA45 | SDpSTSGLSNDSDIFDVIR | S4 | No | Yes | |
| HA72 | LAIpSDFNVDAADLSNNTDDSSTK | S26 | No | Yes | NEK6 (L . . p[ST]) |
| pSILGHNDLTSYR | S46 | No | Yes | CK1 (S . . p[ST], [ST] … pS) | |
| HA74 | HYpSVTASAASK | S228 | No | Yes | GSK3 (pS … S), CAMK2 (R . . p[ST], R . . p[ST]V) |
| VP91 | FVEQYGpTNIHK | T605 | No | Yes | |
| PIF2 | YFAGPQNMpSQVAGR | S171 | No | Yes | DNA damage response kinase (p[ST]Q) |
| NTFRpSHWDELLPDGTR | S210 | No | Yes | ||
| VTHIVPGDKTpSMCAAVVDR | T290 | No | Yes | ||
| F protein | pSKDpSFDQYDEHKL | S665 | Yes | No | |
| S668 | Yes | No | CK1 (S . . p[ST]) | ||
a
ND, not detectable.
b
PKA, protein kinase A; CDK2, cyclin-dependent kinase 2; ERK, extracellular signal-related kinase; CDK1, cyclin-dependent kinase 1; CAMK2, Ca2+/calmodulin-dependent protein kinase; CK1, casein kinase 1; PKD, protein kinase; CHK1, checkpoint kinase protein 1; NEK6, NIMA-related kinase 6; GSK3, glycogen synthase kinase 3; AKT, protein kinase B.