Fig 1.

Normalized proteome view associated with vaccination using Dryvax, Acam2000, or two-dose Imvamune in humans and prairie dogs. The proteins are sorted by function and location (if known), with signal intensities represented by pale green for no response and red for increasingly intense reactions. Average raw values for naïve human and prairie dog serum profiles were included to provide visual controls for background proteome-wide antibody reactivity, whereas VIG and the remaining sample profiles are shown after subtraction of naive reactivity. Each column is an average of measurements for individual vaccinees, with the time point postvaccination indicated above each column. The normalization procedure for comparing human and prairie dog responses is described in Materials and Methods.