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Activity of 50-92 with untagged or FLAG-tagged PB2. 293T cells were transfected with pCAGGS 50-92 NP, PB1, PA, and WT (A) or E627K (B) PB2 (untagged or FLAG tagged), as well as a virus-like firefly luciferase reporter plasmid and pCAGGS Renilla. Firefly luciferase production was measured 12 h posttransfection. Values were normalized to Renilla expression and to the activity of the untagged polymerase. Results shown are the means ± standard deviations from three independent experiments.
