Fig 5.

Relative transmission of Env-chimeric virus from migratory cells emigrating from genital explants to CD4⁺ T cells. Genital tissue explants were exposed to either acute or chronic Env-chimeric virus for 2 h and then washed to remove unbound virus. Migratory cells emigrating from tissue explants were harvested after overnight culture and cocultured with PM-1 CD4 T cells for 21 days. p24 levels were measured in collected culture supernatant by ELISA. Horizontal bars represent the median p24 level for each tissue type. Cervix - A and Penile - A are explants infected with acute Env-chimeric virus, and Cervix - C and Penile - C are explants infected with chronic Env-chimeric virus. Values that were significantly different (P < 0.0001) by the Mann-Whitney t test and using 95% CI are indicated by an asterisk and thin black line.