Fig 3.
CD1d is highly expressed in adipocytes. Tissues were obtained from 8-week-old C57BL6/J male mice. (A) CD1d mRNA expression levels in thymus (THY), spleen (SPN), liver (LIV), epididymal adipose tissue (WAT), kidney (KID), lung (LNG), intestine (INT), and heart (HRT) were detected by Northern blotting. (B) qRT-PCR data of CD1d mRNA levels in each tissue; (C) CD1d mRNA expression level in fat depots; (D) CD1d mRNA expression levels in intraperitoneal macrophages (ip-MACs), SVCs, and adipocytes (AD); (E) mRNA levels of CD1d and PPARγ during 3T3-L1 adipocyte differentiation. (A to E) CT values of CD1d mRNA were around 23, 23, and 26 in adipose tissues, primary adipocytes, and 3T3-L1 adipocytes, respectively. (F) Localization of CD1d in adipocytes, assessed by histological analysis of epididymal adipose tissue. CD1d (red), perilipin (green), and DAPI (blue) are seen. (G) mRNA levels of CD1d, PPARγ, and aP2 in CD1d overexpressing stable cells; (H) mRNA levels of CD1d, PPARγ, and aP2 in CD1d short hairpin RNA (shRNA)-overexpressing stable cells; (I) oil red O staining at day 8 of adipogenesis. *, P < 0.05; **, P < 0.01; ***, P < 0.001.