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. 2013 Jan;33(2):252–264. doi: 10.1128/MCB.00976-12

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Fig 5 — dSir2 in the fat body and muscles regulates glucose homeostasis. An oral glucose tolerance test (oGTT) was performed using hemolymph isolated from pSw-MHC-Gal4/Sir2^EP2300 (mdSir2^OE) (A), pSw-MHC-Gal4; UAS-Sir2^RNAi (mdSir2^KD) (B), pSw-S₁106-Gal4/Sir2^EP2300 (fbdSir2^OE) (C), and pSw-S₁106-Gal4; UAS-Sir2^RNAi (fbdSir2^KD) (D) flies. For every time point, 5 samples from hemolymph isolated from 12 flies each were used. Control, −RU486; overexpression/knockdown, +RU486 (200 μM). All data are shown as means ± standard errors of the means. *, P < 0.05; **, P < 0.01; ***, P < 0.001.