Fig 5.

The EJC subunits required for the nuclear retention of unspliced RNAs. (A) RT-PCR assays were performed to monitor the expression of tra-2 and ama-1 in the animals subjected to RNAi as indicated. The primers used for amplification are schematically shown on the left. Arrowheads indicate unspliced RNAs. (B) In situ hybridization of mitotic cells within gonad arms dissected from adult hermaphrodites subjected to RNAi as indicated. Cells were probed with tra-2 intron 20 (green), followed by DNA staining (magenta), shown as merged views (top). Separate views of single cells probed with tra-2 intron 20 are also shown (middle). The intracellular distribution of the intron is shown as described in the legend to Fig. 3B (bottom). Scale bars, 4 μm.