Fig 2.

Characterization of Gαq-mediated signaling of the wild-type TP and chimeric receptors. Receptor activity was determined by measuring the agonist-independent and -dependent changes in intracellular calcium levels using transiently transfected HEK293T cells. The data show the basal (zero concentration of agonist or water alone) and agonist U46619-induced calcium mobilization for the WT TP, chimeric receptors, and mock-transfected (vector pMT4) HEK293T cells. The results are expressed as a percentage of the WT TP activity and are from at least three independent experiments performed in duplicate. The results were normalized to calcium mobilized (ΔRFUs) relative to cell surface expression of the receptors as determined by FACS.