Fig 5.

FOXM1 recruitment depends on the MMB complex. (A) ChIP assay of endogenous FOXM1 was performed in U2OS cells on the promoters of the indicated genes after knockdown of LIN9 or in the presence of a nontargeting control siRNA (siCon). (B) Western blot of FOXM1 and LIN9 levels after knockdown of LIN9 or in the presence of a nontargeting control siRNA (siCon). ERK2 represents a loading control. (C) ChIP assays of transiently transfected Flag-tagged FOXM1 in HEK293T cells. FOXM1 ChIP was performed on promoters of the indicated genes after knockdown of LIN9 or B-MYB or in the presence of a nontargeting control siRNA (siCon). “Vec” represents cells transfected with empty vector. The data for both ChIP experiments are the average of two independent experiments. ** and * represent P values of <0.01 and <0.05, respectively. (D) RT-qPCR analysis of the expression of the indicated genes in asynchronously growing U2OS cells. Cells were treated with a nontargeting siRNA (siCon) or a siRNA against LIN9. The data are the average of two experiments and are shown for each gene relative to its expression in the presence of the control siRNA (taken as 1). ** and * represent P values of <0.001 and <0.01, respectively.