Fig 5.

The V1068G mutation leads to loss of DNA binding. (A and B) Single-component saturating EMSAs (ssEMSAs) using titrated amounts of wild-type (wt) or mutant (mut; V → G) recombinant protein fragments containing the non-sequence-specific ARID domain of H. sapiens (Hs) ARID1a. (D and E) Similar ssEMSAs using titrated amounts of wild-type or mutant (V → G) recombinant protein fragments containing the sequence-specific ARID domain of D. melanogaster Retn/Dri/Bright. Subsaturating amounts of the dri.16 probe (50 pM) were used in each DNA binding reaction. (C and F) Fractional occupancies were calculated from band densitometry measurements and plotted versus log protein concentrations. Blue dotted lines in panels C and F represent curves fitted to EMSA results using Kaleidagraph software.