Fig 4.

Senataxin foci increase upon replication stress and colocalize with 53BP1 and H2AX. (a) Cells were treated with 0.4 μM aphidicolin for 24 h, fixed, and immunostained with the indicated antibodies. (Top) Cells taken at different mitotic stages. (Bottom) Costaining with cyclin A indicates that replication stress induces senataxin foci. (b) Quantification of senataxin foci in S/G₂-phase HeLa cells (n = 120) treated with or without aphidicolin. (c and d) Cells were treated as described for panel a, and senataxin foci were colocalized with 53BP1 and H2AX.