Fig 7.

In vitro and in vivo activities of hybrid CIRV genomes containing 3′CITE mutations obtained during serial passage. (A) Graphical representation of p36 accumulation in wge translation reactions. The relative levels of p36 produced from the RNAs identified below the graph were normalized to the level seen with CIRV-ΔTE (set to 1). (B) Accumulation of viral genomic RNAs in N. benthamiana and barley protoplasts (analyzed by Northern blotting). (C) Upper panel: inoculated leaves at 6 dpi and upper noninoculated leaves at 6 dpi (for CIRV, CIRV-C, CIRV-Cp, and CIRV-CpTE) or 10 dpi (for mock treatment, CIRV-M, CIRV-Mp, and CIRV-MpTE). Lower panel: Northern blot showing viral RNA accumulation in leaves from two plants inoculated with the RNAs indicated above the photographs. Leaves shown are representative of all plants inoculated with the indicated genome over three separate experiments. In each trial, two plants were inoculated with each genome.