Fig 10.

(A) IRF7 inhibits EV71 replication. RD cells were transfected with IRF7 variants and IRF3 as indicated. At 24 h after transfection, cells were treated with EV71. After 24 h, cells were harvested and resolved with 12% SDS-PAGE. Western blot analysis for VP1, Myc, or β-actin was conducted. (B) Densitometry analysis. VP1 protein bands from three independent experiments in panel A were quantified and normalized to β-actin by using the Odyssey image software. (C) The effects of IRF7 on viral RNA synthesis. RD cells were transfected with IRF7, 189N, 189C, or IRF3 for 24 h. Cells were infected with EV71, and at 24 h postinfection, the total RNA extracted from the cells were analyzed for the expression of EV71 VP4 RNA. GAPDH was used as an internal control. (D) IRF7 is cleaved in EV71-infected cells. 293T cells were transfected with IRF7 for 24 h. Cells were then mock infected or infected with an increasing dose of EV71. At 24 h after infection, cell lysates were analyzed by Western blotting using antibodies against Myc, EV71, and β-actin.