Fig 5.

The effect of selected inhibitors on IRF7 cleavage. 293T cells were transfected with Myc-Flag-IRF7 along with GFP or GFP-3C. At 24 h after transfection, cells were incubated with the protease inhibitor rupintrivir (2 μM) (A) (31), caspase inhibitor Z-VAD (20 μM) (B) (6, 32), lysosome inhibitor NH₄Cl (10 mM) (C) (33), proteasome inhibitor MG132 (0.1 μM) (D) (33), and autophagy inhibitor 3-MA (500 μM) (E) (34) for 24 h. Cell lysates were then processed for Western blot analysis.